Exploiting the Potential of Magnetic Nanoparticles for Rapid Diagnosis Tests (RDTs): Nanoparticle-Antibody Conjugates and Color Development Strategies

Magnetic nanoparticles (MNPs) have emerged as a promising material in disease diagnostics due to their potential enhance detection sensitivity, facilitate concentration and purification of target substances diverse samples, enable favorable color-based detection. In this study, antibody-conjugated MNPs were successfully synthesized validated through two appropriate methods: the measurement MNPs’ size use phosphatase methods. Additionally, three methods suggested implemented for developing color MNPs-based immunoassay, including formation MNP aggregations, utilization peroxidase-like activity, synthesis dually-conjugated with both enzyme antibody. particular, development utilizing nanoparticle aggregations was demonstrated result more yellowish virus increased, while peroxidase activity exhibited proportional increase intensity increased. This observation suggests applicability quantitative analysis using these Furthermore, effective collection an external magnetic field, irrespective factors such antibody conjugation, dispersion medium, or binding. Finally, based on key findings design proposal immunoassay is presented. Overall, immunoassays hold significant advancing diagnostics.